Supercritical fluid extraction
Supercritical fluid extraction (SFE) is to extract pigments from microalgae using supercritical carbon dioxide instead of an organic solvent. The supercritical fluid state is when a fluid is above its critical temperature and critical pressure when it is between the typical gas and liquid state. Then it will manipulate the temperature and pressure of the sample which can solubilize the sample and will proceed to extraction. The sample will be in extraction vessel and CO2 will be pressurized only to dissolve the sample. Then the sample is transferred to fraction collector to get depressurized so that the material is to precipitate.